Jiang, H., & Mei, Y. F. (2021). SARS-CoV-2 Spike Impairs DNA Damage Repair and Inhibits V(D)J Recombination In Vitro. Viruses, 13(10), 2056. doi.org/10.3390/v13102056 (Retraction published Viruses. 2022 May 10;14(5):)viruses-13-02056-v3
PMID- 34696485 OWN - NLM STAT- MEDLINE DCOM- 20211110 LR - 20220528 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 13 IP - 10 DP - 2021 Oct 13 TI - SARS-CoV-2 Spike Impairs DNA Damage Repair and Inhibits V(D)J Recombination In Vitro. LID - 10.3390/v13102056 [doi] LID - 2056 AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to the coronavirus disease 2019 (COVID-19) pandemic, severely affecting public health and the global economy. Adaptive immunity plays a crucial role in fighting against SARS-CoV-2 infection and directly influences the clinical outcomes of patients. Clinical studies have indicated that patients with severe COVID-19 exhibit delayed and weak adaptive immune responses; however, the mechanism by which SARS-CoV-2 impedes adaptive immunity remains unclear. Here, by using an in vitro cell line, we report that the SARS-CoV-2 spike protein significantly inhibits DNA damage repair, which is required for effective V(D)J recombination in adaptive immunity. Mechanistically, we found that the spike protein localizes in the nucleus and inhibits DNA damage repair by impeding key DNA repair protein BRCA1 and 53BP1 recruitment to the damage site. Our findings reveal a potential molecular mechanism by which the spike protein might impede adaptive immunity and underscore the potential side effects of full-length spike-based vaccines. FAU - Jiang, Hui AU - Jiang H AD - Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-10691 Stockholm, Sweden. AD - Department of Clinical Microbiology, Virology, Umeå University, SE-90185 Umeå, Sweden. FAU - Mei, Ya-Fang AU - Mei YF AD - Department of Clinical Microbiology, Virology, Umeå University, SE-90185 Umeå, Sweden. LA - eng GR - 3453 16032,ALF-Basenhet 2020, 2021, AMP 19-982, and LP 20-2256/Umeå University, ALF-Basenhet,the Lion's Cancer Research Foundation/ PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Retracted Publication DEP - 20211013 TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Antibodies, Neutralizing) RN - 0 (Antibodies, Viral) RN - 0 (BRCA1 Protein) RN - 0 (BRCA1 protein, human) RN - 0 (COVID-19 Vaccines) RN - 0 (Spike Glycoprotein, Coronavirus) RN - 0 (TP53BP1 protein, human) RN - 0 (Tumor Suppressor p53-Binding Protein 1) RN - 0 (spike protein, SARS-CoV-2) SB - IM ECI - Viruses. 2021 Dec 22;14(1):. PMID: 35062216 RIN - Viruses. 2022 May 10;14(5):. PMID: 35632859 MH - Adaptive Immunity/*immunology MH - Antibodies, Neutralizing/blood/immunology MH - Antibodies, Viral/blood/immunology MH - BRCA1 Protein/antagonists & inhibitors MH - CD4 Lymphocyte Count MH - CD8-Positive T-Lymphocytes/immunology MH - COVID-19/*pathology MH - COVID-19 Vaccines/immunology MH - Cell Line MH - DNA Damage/genetics MH - DNA Repair/*genetics MH - HEK293 Cells MH - Humans MH - Immunity, Humoral/immunology MH - Immunosuppression Therapy MH - SARS-CoV-2/genetics/*immunology MH - Spike Glycoprotein, Coronavirus/*genetics/immunology MH - T-Lymphocytes, Helper-Inducer/immunology MH - Tumor Suppressor p53-Binding Protein 1/antagonists & inhibitors MH - V(D)J Recombination/*genetics PMC - PMC8538446 OTO - NOTNLM OT - *DNA damage repair OT - *SARS–CoV–2 OT - *V(D)J recombination OT - *spike OT - *vaccine COIS- The authors have declared that no competing interests exist. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. EDAT- 2021/10/27 06:00 MHDA- 2021/11/11 06:00 CRDT- 2021/10/26 01:03 PHST- 2021/08/20 00:00 [received] PHST- 2021/09/08 00:00 [revised] PHST- 2021/10/08 00:00 [accepted] PHST- 2021/10/26 01:03 [entrez] PHST- 2021/10/27 06:00 [pubmed] PHST- 2021/11/11 06:00 [medline] AID - v13102056 [pii] AID - viruses-13-02056 [pii] AID - 10.3390/v13102056 [doi] PST - epublish SO - Viruses. 2021 Oct 13;13(10):2056. doi: 10.3390/v13102056.